Single Channel or Dual Channel Open qPCR?

Understanding the differences between the single channel and dual channel open qPCR instruments will allow you to decide which instrument is the best option for your needs. The number of target sequences your assay design needs to amplify and the fluorophores required by your test kit are important to consider, along with the desired level of monetary investment.

Single Channel Open qPCR 

• One channel
• One target sequence detection/reaction tube
• 16 wells
• Wavelength detection range: 513 nm – 555 nm

Single channel open qPCR instruments are a great choice when minimizing cost is your top concern. However, it is important to keep in mind that a single channel instrument will significantly limit the capabilities of your assay, as detection for only one target sequence may be performed in each reaction tube.

Dual Channel Open qPCR

• Two channels
• Two target sequence detection/reaction tube
• 16 wells
• Wavelength detection ranges: 508 nm – 532 nm & 573 nm – 597 nm

Dual channel open qPCR instruments are ideal for any operation wishing for multiplexing capabilities, which offers many advantages. The two channels in these instruments allow for detection of two target sequences in each reaction tube.  If your assay requires amplification of multiple target sequences, using multiplex PCR rather than several singleplex PCR reactions is much more efficient, as it maximizes the use of limited starting material and lowers reagent costs. Instruments with multiplexing capabilities are ideal for high-volume environments that need to perform large quantities of the same test repeatedly. Additionally, a multiplexed channel may be used as an internal control for your assay to confirm the absence of reaction inhibitors or verify the completion of the extraction process.