The Superattenuator Yeasts Test Kit detects Diastaticus and Brettanomyces and is designed for direct qPCR. This typically requires a 50 mL spindown of your sample:
- Centrifuge samples for 5 minutes at 2400 g or higher (note that RPMs required for 2400 g will vary by centrifuge).
- Decant the supernatant (liquid portion), leaving about 1 mL in the cone of the tube.
- Mix remaining solution in the cone with a pipette tip and transfer it to a new 1.5 mL tube.
- Proceed with the next step in sample prep.
Alternatively, to increase sensitivity for Diastaticus and Brettanomyces, separate enrichments may be performed prior to running the test kit by enriching samples separately in FastOrange Brett Broth for Brettanomyces and FastOrange Yeast Broth for Diastaticus. Combine both resulting enrichment samples into a single 1.5 mL tube and run with the Superattenuator Yeasts Test Kit. Note that the recommended enrichment time for Diastaticus is 48 hours while Brettanomyces is 72-96 hours.
You can also perform a single enrichment for Diastaticus or Brettanomyces, then run the test kit. In this case, you'll be testing for one spoiler with a high limit of detection and the other with a degree of sensitivity more similar to direct qPCR.